WebPublication (1) Datasheet SDS. Tested applications: WB Reactivity: Human, Mouse, Rat. Western blot analysis of extracts of various cell lines, using CLPB antibody (A9130) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. WebPrimePCR™ Template for Probe Assay: CLPB, Human Reaction: 200 x 20 µl reactions Gene-specific synthetic DNA template designed to give a positive real-time PCR result …
Gel filtration analysis of ClpB and its truncated variants in the ...
WebWe conclude that ClpB Δ C binds ATP with a low affinity, most likely with K d > 1 mM. We tested the ClpB variants in a luciferase reactivation assay that had been previously used … WebAug 27, 2003 · Small heat shock proteins (sHsps) can efficiently prevent the aggregation of unfolded proteins in vitro.However, how this in vitro activity translates to function in vivo is poorly understood. We demonstrate that sHsps of Escherichia coli, IbpA and IbpB, co-operate with ClpB and the DnaK system in vitro and in vivo, forming a functional triade of … folding stairs for boats
Filter Binding Assay - an overview ScienceDirect Topics
WebFeb 7, 2013 · The 580-kD hexameric ClpB molecular chaperone is a bacterial adenosine 5′-triphosphate (ATP)–dependent protein-remodeling machine that rescues stress-damaged proteins trapped in an aggregated state and plays a key role in thermotolerance development and in cell recovery after acute stress (1–3).Aggregate reactivation requires … WebJan 30, 2015 · To identify the site on E. coli DnaK that interacts with ClpB, we substituted amino acid residues throughout the DnaK NBD. We found that several variants with … WebSep 1, 2006 · ClpB ATPase assay. ClpB samples were incubated for 15 min at 37 °C in 100 mM Tris–HCl, pH 8, 10 mM MgCl 2, 5 mM ATP, 1 mM EDTA, 1 mM DTT without, or with 0.1 mg/ml κ-casein (Sigma). Inorganic phosphate production was determined using the malachite green method , . Urea-induced unfolding of ClpB egyptian goddess of truth justice and harmony