WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ...
PhosphoPair mTOR (Ser2448) Antibody Set, mTOR, 6H9B10; …
WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … WebUnc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. chiropractor buda texas
Anti-phospho-mTOR (Ser2159) Antibody ABS79 - EMD Millipore
WebAnti-mTOR (phospho T2446) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho T2446) Host species Rabbit Tested applications Suitable for: WB more details Species reactivity Reacts with: Mouse Immunogen Synthetic peptide corresponding to Human mTOR (phospho T2446). Database link: P42345 General notes WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … Webindicates those antibodies for which a 10 µg trial size SAMPLE is available free of charge. indicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. mTOR Antibodies Phospho-Specific mTOR Antibodies mTOR CRISPR Knockout, HDR and Nickase Knockout Plasmids mTOR CRISPR Activation … graphics card rtx 4070